Singkil leaves (Premna corymbosa) empirically contain various secondary
metabolite compounds, one of which is flavonoids that can be used as asthma
medication, antitumor, worm infection, and reduce bad breath. Drying is one of
the stages of making simplicia which is done so that simplicia is not damaged.
This study aims to determine the effect of drying method on total flavonoid
content in singkil leaf extract.
The research method used is experimental. The research phase includes
sampling, plant determination, drying simplicia with oven temperature of 50oC
drying andin an open space and protected from indirect sunlight, extraction is
done by maceration, phytochemical screening, determination of total flavonoid
content. The assay method used was colorimetry using UV-Vis
spectrophotometer with AlCl3 reagent and quercetin standard. The calibration
curve was made for 5 concentrations, namely 12,5; 15; 17,5; 20; and 22,5 ppm.
The absorbance was calculated at a wavelength of 415 nm and the reading was
used as a determinant of the total flavonoid content of the singkil leaf extract.
The results showed that the drying methods may affect the levels of total
Singkil leaf extract significantly, with higher levels of total flavonoids extract of
leaves of Singkil using keringangin of 2.27 ± 0.02%, while the total content of
flavonoids extract of leaves of Singkil using the oven 50oC for 1.50±0.02%.
Keywords : singkil leaf (Premna corymbosa), total flavonoid levels,method
drying, AlCl3, quercetin