Singkil leaves (Premna corymbosa) contain secondary metabolites in
the form of flavonoids, saponins, triterpenoid/steroid tannins and phenolic
compounds. Phenol compounds have antioxidant, antitumor, antiviral and
antibiotic activities. The drying process of simplicia will have an impact on the
content of phenolic compounds. This study aims to determine the effect of the
drying method on the total phenolic compounds in singkil leaf extract.
The type of research that will be conducted is experimental. Singkil leaf
simplicia drying method was carried out using an oven at a temperature of 50°C
and air-dried. The extraction method uses maceration with 70% ethanol as
solvent. Determination of total phenolic content was carried out by colorimetric
method using UV-Vis spectrophotometry with Follin Chiocalteau reagent and
gallic acid as standard. The calibration curve was made in 5 concentration series,
namely 6, 8, 10, 12, and 14 ppm. The absorbance was calculated at a
wavelength of 278.65 nm and the readings were used as a determinant of the
total phenolic content of the singkil leaf extract.
The results showed that the simplicia drying method could significantly
affect the total levels of singkil leaf extract, with the total flavonoid content of
singkil leaf extract using the wind dry method of 13.37±0,0% while the total
flavonoid content of singkil leaf extract using the 50˚C oven drying method was
10.64±0,00057%. .
Keywords: Premna corymbosa Rottl et Willd, total phenolic compounds content,
drying method, Gallic Acid, Follin Chiocalteu.